Glutathione (GSH) homeostasis and dendritic arbor are both key biomarkers for neurodegeneration. Therefore, with an aim of understanding the linkage between neurodegeneration, these two indicators, and a neuron’s health, we have investigated the correlation between the dendritic arborization of a neuron and its corresponding ratio between GSH and oxidized glutathione (GSSG). Additionally, while previous studies have focused on a macro perspective of this process, we observed these neurites at a subcellular scale using gradient light interference microscopy (GLIM) and confocal laser-scanning microscopy. From there, the program FIJI, was used to run Sholl analysis and to analyze the GSH:GSSG ratios of collected images. We believe that our study has the potential to provide a new perspective on understanding the varying levels of arborization in cultured neurons and elucidate the underlying mechanisms behind neurodegeneration.